Basics Chemically comp. cells - Inoue Chemically comp. cells - RbCl Electrocompetent cells Molecular cloning Media recipes Transformation Affinity bead regeneration Plasmid purification Gels Stocks Protein purification Gb1 purification TEV purification protocol TEV expression/purification detailed (AY) Ulp1 purification Ulp1 expression/purification detailed (SR) Isotopic labeling 15N-labeling Genetic code expansion pSer incorporation nhpSer incoporation NMR